Erythroid differentiation and maturation from peripheral CD34+ cells in liquid culture: cellular and molecular characterization
L Ronzoni, P Bonara, D Rusconi, C Frugoni… - Blood Cells, Molecules …, 2008 - Elsevier
In vitro models of human erythropoiesis are useful in studying the mechanisms of erythroid
differentiation from BFU-E to mature erythrocytes both in normal and pathological conditions.
Most of the available in vitro liquid cultures are from cell lines or are limited by the production
of few erythroid cells mixed with myeloid cells. Here we describe an erythroid liquid culture
system starting from CD34+-enriched cells obtained from peripheral blood. CD34+ cells
were cultured for 21 days in different conditions. Precisely stem cell factor (SCF, 20 ng/mL) …
differentiation from BFU-E to mature erythrocytes both in normal and pathological conditions.
Most of the available in vitro liquid cultures are from cell lines or are limited by the production
of few erythroid cells mixed with myeloid cells. Here we describe an erythroid liquid culture
system starting from CD34+-enriched cells obtained from peripheral blood. CD34+ cells
were cultured for 21 days in different conditions. Precisely stem cell factor (SCF, 20 ng/mL) …
