Accurate quantification of mouse mitochondrial DNA without co-amplification of nuclear mitochondrial insertion sequences
AN Malik, A Czajka, P Cunningham - Mitochondrion, 2016 - Elsevier
Background Mitochondria contain an extra-nuclear genome in the form of mitochondrial
DNA (MtDNA), damage to which can lead to inflammation and bioenergetic deficit. Changes
in MtDNA levels are increasingly used as a biomarker of mitochondrial dysfunction. We
previously reported that in humans, fragments in the nuclear genome known as nuclear
mitochondrial insertion sequences (NumtS) affect accurate quantification of MtDNA. In the
current paper our aim was to determine whether mouse NumtS affect the quantification of …
DNA (MtDNA), damage to which can lead to inflammation and bioenergetic deficit. Changes
in MtDNA levels are increasingly used as a biomarker of mitochondrial dysfunction. We
previously reported that in humans, fragments in the nuclear genome known as nuclear
mitochondrial insertion sequences (NumtS) affect accurate quantification of MtDNA. In the
current paper our aim was to determine whether mouse NumtS affect the quantification of …
